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Advances in Polymer Technology , 36 4 , Solvent-responsive floating liquid crystalline-molecularly imprinted polymers for gastroretentive controlled drug release system. International Journal of Pharmaceutics , 1 , Molecularly imprinted polymer for glutathione by modified precipitation polymerization and its application to determination of glutathione in supplements. Journal of Pharmaceutical and Biomedical Analysis , , Synthesis and application of molecularly imprinted polymers for the selective extraction of organophosphorus pesticides from vegetable oils. Enantioselective determination of S -ornidazole by using carbon paste electrode modified with boron-embedded conductive copolymer-polysiloxane-based molecularly imprinted hybrid film.

Electrochimica Acta , , Preparation of molecularly imprinted polymers for warfarin and coumachlor by multi-step swelling and polymerization method and their imprinting effects. Journal of Rare Earths , 35 7 , Towards a new strategy of a chitosan-based molecularly imprinted membrane for removal of 4-nitrophenol in real water samples.


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Polymer International , 66 7 , Anirudhan, J. Christa, J. Extraction of melamine from milk using a magnetic molecularly imprinted polymer. Food Chemistry , , Jager, Valerio Beni, Anthony P. Electrochemical bacterial detection using poly 3-aminophenylboronic acid -based imprinted polymer. Biosensors and Bioelectronics , 93 , Sarzamin Khan, Eliane M. Miguel, Cabrini F.

Molecularly Imprinted Polymers (MIPs)

Thioglycolic acid-CdTe quantum dots sensing and molecularly imprinted polymer based solid phase extraction for the determination of kanamycin in milk, vaccine and stream water samples. Angewandte Chemie , 25 , Angewandte Chemie International Edition , 56 25 , Specific rebinding of protein imprinted polyethylene glycol grafted calcium alginate hydrogel with different crosslinking degree.

Electrochemical microfluidic chip based on molecular imprinting technique applied for therapeutic drug monitoring. Biosensors and Bioelectronics , 91 , Neeraj Kumar, Rosy, Rajendra N. A melamine based molecularly imprinted sensor for the determination of 8-hydroxydeoxyguanosine in human urine. Giulia Selvolini, Giovanna Marrazza. Sensors , 17 4 , Chunhua Zhou, Mona A. Thermoresponsive dendronized copolymers for protein recognitions based on biotin—avidin interaction. Chinese Chemical Letters , 28 4 , Poly ethylene glycol acrylate -functionalized hydrogels for heparan sulfate oligosaccharide recognition.

Journal of Molecular Recognition , 30 3 , e Chemosensors , 5 1 , 7. May A. Abd El-Aal, Medhat A. Preparation and characterization of pH-responsive polyacrylamide molecularly imprinted polymer: Application to isolation of recombinant and wild type human serum albumin from biological sources.

Journal of Chromatography B , , Development of novel electrochemical sensor on the base of molecular imprinted polymer decorated on SiC nanoparticles modified glassy carbon electrode for selective determination of loratadine. Materials Science and Engineering: C , 71 , Mariana I. Neves, Marissa E. Wechsler, Manuela E. Gomes, Rui L. Reis, Pedro L. Granja, Nicholas A. Tissue Engineering Part B: Reviews , 23 1 , Jevan Medlock, Anupam A.

Das, Leigh A. Madden, David J. Allsup, Vesselin N. Cancer bioimprinting and cell shape recognition for diagnosis and targeted treatment. Chemical Society Reviews , 46 16 , Journal of Separation Science , ,, Romana Schirhagl. Bacteria-templated fabrication of a charge heterogeneous polymeric interface for highly specific bacterial recognition.

Chemical Communications , 53 15 , Journal of Materials Chemistry B , 5 5 , Uichi Akiba, Jun-ichi Anzai. Recent Progress in Electrochemical Biosensors for Glycoproteins. Sensors , 16 12 , Surface plasmon resonance sensor for antibiotics detection based on photo-initiated polymerization molecularly imprinted array.

Zeynep Altintas. Advanced Imprinted Materials for Virus Monitoring. Sunil Kr. Selective extraction of proteins and other macromolecules from biological samples using molecular imprinted polymers. Bioanalysis , 8 21 , Angewandte Chemie International Edition , 55 42 , Angewandte Chemie , 42 , Core-shell nanoparticles coated with molecularly imprinted polymers: a review. Microchimica Acta , 10 , Molecularly imprinted polymers synthesized using reduction-cleavable hyperbranched polymers for doxorubicin hydrochloride with enhanced loading properties and controlled release.

Journal of Materials Science , 51 20 , Oliveira, C. Gomes, R. Dias, M. Molecular imprinting of 5-fluorouracil in particles with surface RAFT grafted functional brushes. Reactive and Functional Polymers , , Gerhard K. Chiral recognition in separation science — an update.

Judith Wackerlig, Peter A. Polymers, Molecularly Imprinted. Alvaro V. Gutierrez R. Bioimprinting as a tool for the detection of aflatoxin B1 using a capacitive biosensor. Biotechnology Reports , 11 , Sensors , 16 9 , Molecularly imprinted intelligent scaffolds for tissue engineering applications. The goal of this title is to capture this momentum and publish a new book that will reflect the current situation in this rapidly evolving technology.

Molecularly Imprinted Polymers in Biotechnology

Very few of the tens of reviews already published on this subject present a critical analysis of the technological aspects of molecular imprinting. Leaders in this field have been approached with requests to provide their views and analyses of specific areas of design, characterization and application of these polymers. The last, but by no means least, part of the book is dedicated to often overlooked associated aspects of MIPs such as commercialization strategy and IPR, prepared by Peter Leverkus and Jeffrey McIntyre.

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Fulfilment Centre Email: siamparagon kinokuniya. Check Retail Stores' phone number. Data were graphically plotted and statistically analyzed using GraphPad Prism v7. Datasets for other assays were technical replicates within single experiments. Whilst infectious virus was detected in all sucrose gradient fractions, the highest titers were detected, as predicted, in the central fractions of the gradient, i.

Testing of the individual sucrose gradient fractions confirmed the highest titers in fractions 16—18 Figure 1B and these fractions were dialyzed into PBS and inactivated prior to imprinting. Infectious virus titers post-dialysis and post-inactivation were determined data not shown and electron microscopy confirmed the structural integrity of the template virus and the absence of significant cellular debris Figure 1C.

A PBS control treatment was used as a further negative control in each assay. There was no inter-batch variation in this effect suggesting a reproducible imprinting-related neutralization of virus. However, pNIPAM produced a very similar reduction in infectious viral titer in both its MIP and NIP forms, suggesting that molecular imprinting was not entirely responsible for the neutralization event in the case of this polymer. Figure 1.

Template virus was prepared by purification of PRRSV-1 virions by continuous density ultracentrifugation. Pools of gradient fractions A and individual fractions B containing the highest infectious titers were selected and virion purity and integrity post-dialysis and inactivation confirmed by electron microscopy C. The limit-of-detection of the assays are indicated by dashed horizontal lines.

Both MIPs showed a broadly similar effect with decreasing concentration, providing complete neutralization of infectious virus to the LoD of the assay at both neat and dilutions. Figure 2. Characterization of the virus neutralizing properties of hydrogel MIPs. The limit-of-detection of the assays are indicated by horizontal dashed lines. This is the first study to demonstrate that MIPs imprinted with a clinically relevant virus can exert potent antiviral effects, reducing the infectious viral titer recovered to below the LoD of the assay used.


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  • The concentration and time dependent effect of neutralization further supports a rapid and specific binding. That there is a virus neutralization event in this study, regardless of imprinting taking place, points to the potential toxic nature of pNIPAM. It has been established in this study with an animal virus and by others with bacteriophages Sankarakumar and Tong, ; Li et al.

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    Molecularly Imprinted Polymers in Biotechnology by Bo Mattiasson, Paperback | Barnes & Noble®

    The results of our incubation time trial showing that complete neutralization could be achieved in as little as 2. However, this would need to be re-assessed in the context of plasma proteins and other potentially interfering molecules that would be present in vivo. In terms of suitability for in vivo testing, further work is needed to ensure suitable biocompatibility of our MIPs. Li et al. The latter demonstrated the ability of MIPs to bind the cytotoxic peptide melittin, the principle component of bee venom, in the bloodstream of mice, which significantly reduced the mortality and morbidity associated with melittin envenomation.

    The data from this study has demonstrated a highly effective and specific neutralization of virus infectivity with certain hydrogel-based MIPs. Whilst promising, it is possible that the destructive method used to produce these cavity-containing hydrogel-MIPs leads to the majority of the material comprising redundant unselective particles, devoid of template-specific cavities.

    Further studies will evaluate virus imprinting of nanoparticle-based MIPs nanoMIPs for the efficient production of high bioaffinity materials Canfarotta et al. MIPs imprinted with virions may be produced according to a variety of methods, giving nanoscale shells with cavity populated surfaces. In conclusion, hydrogel-based MIPs are capable of specifically neutralizing virus infectivity in vitro within a short enough incubation time to be clinically relevant. All authors contributed to manuscript revision, read, and approved the submitted version.

    The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

    Ahmad, O. Molecularly imprinted polymers in electrochemical and optical sensors. Trends Biotechnol. Altintas, Z. Biosensors for waterborne viruses: detection and removal. Biochimie , — Canfarotta, F. Solid-phase synthesis of molecularly imprinted nanoparticles. Recent advances in electrochemical sensors based on chiral and nano-sized imprinted polymers. El-Sharif, H. Actuators B Chem. Determination of protein binding affinities within hydrogel-based molecularly imprinted polymers HydroMIPs.

    Enhanced selectivity of hydrogel-based molecularly imprinted polymers HydroMIPs following buffer conditioning.

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    Acta , — Finney, D. Statistical Method in Biological Assay, 3rd edn. Google Scholar. Hawkins, D. Investigation of protein imprinting in hydrogel-based molecularly imprinted polymers HydroMIPs. Acta , 61— Hayden, O. Mass-sensitive detection of cells, viruses and enzymes with artificial receptors. Holtkamp, D. Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers.